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Star Republic: Guide for Biologists

Antibiotic stocks

  • Ampicillin (50 mg/ml) in water, filter sterilize, store in -20 C. Working concentrations 20-125 mg/ml.
  • Kanamycin (25mg/ml) in in water, filter sterilize, store in -20 C. Working concentrations 10-50 mg/ml.
  • Tetracycline (10 mg/ml) in 50% ethanol, store in dark -20 C. Working concentrations 10-50 mg/ml.
  • Chloramphenicol (25 mg/ml) in ethanol, store in -20 C. Working concentrations 25-175 mg/ml.
  • Streptomycin (10 mg/ml) in water, filter sterilize, store in -20 C. Working concentrations 10-50 mg/ml.
  • Carbencillin (50 mg/ml) in water, filter sterilize, store in -20 C. Working concentrations 20-60 mg/ml.
  • Hygromycin B (100 mg/ml) in water, filter sterilize, store in -20 C. Working concentrations 50-100 mg/ml.
1:1000 dilution of the antibiotic stocks is frequently used for liquid media and 1:500 dilution is frequently used for agar plates.