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Star Republic: Guide for Biologists

Establish stable cell clones --- 96-well

Virtualab Protocols (Chang Bioscience)
1. Resuspend transfected cells in culture medium containing appropriate selection antibiotics 48 hours after transfection. Disperse cells into a single-cell suspension by slow pipetting.

2. Dilute cells to different concentrations ranging from 5x103 cells/ml to 105 cells/ml.

3. Dispense into 96-well plate at 200 l/well. Culture at 37 C in a humanitified incubator. Clones should form in 7-14 days.