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Star Republic: Guide for Biologists

Serum starvation

1. Grow cells to subconfluence or desired density in medium containing 5-10% serum (e.g., FCS).

2. Remove medium and wash cells with growth medium/0.2% serum.

3. Incubate cells in growth medium/0.2% serum overnight or a desired length.

4. Stimulate cells with growth factor(s) or normal concentration of serum.