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Star Republic: Guide for Biologists

Quick freezing of cells on plates or flasks

1. Remove media from wells or a tissue culture flask.

2. Add 1X chilled freezing medium to the cells.

1X freezing medium (I): 7%-10% DMSO in growth medium with 10% FCS and without antibiotics
1X freezing medium (II): 7%-10% glycerol in growth medium with 10% FCS without antibiotics

3. Place plates or flasks in a prechilled styrofoam container (-70 C) and close off completely. Place in -70 C freezer. Cells can be stored at -70 C for months.