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Star Republic: Guide for Biologists

Phalloidin staining of filamentous actin

1. Wash cells with PBS.

2. Fix in 2% paraformaldehyde in PBS for 15 minutes at RT.

3. Wash with 10 mM ethanolamine in PBS for 5 minutes.

4. Permeabilize cells in 0.1% Triton-X100 in PBS for 1 minute.

5. Incubate cells in Rhodamine-phalloidin (or FITC-phalloidin) (1:100) in PBS for 15 minutes.

6. Wash in PBS, 3 x 5 minutes.

7. Stain for nuclei. Or mount and view by fluorescent microscopy.