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Star Republic: Guide for Biologists

Mycoplasma test --- visulization of DNA

Mycoplasmas are small (0.2-0.3 m) wall-less bacteria that can grow to very high concentrations in cell cultures. Mycoplasma contamination of cell culture is a common occurrence and diffcult to detect.

PCR Kit such as ATCC Mycoplasma detection kit can be used to detect mycoplasma contamination.

A quick but less sensitive method is to visulize mycoplasma DNA using DAPI or Hoechst dye. Mycoplasma will appear as fluorescent spots in the cytoplasm and on membranes.