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Star Republic: Guide for Biologists

Luciferase activity assay --- 96-well

1. Wash cells transfected with luciferase reporter gene with PBS.

2. Add 100 ml of lysis buffer (25 mM Tris-phosphate pH 7.8, 2 mM DTT, 2 mM CDTA, 10% glycerol, 0.5% Triton X-100).

3. Incubate at room temperature for 20 minutes.

4. Transfer 20 ml of the cell extract to a new 96-well plate.

5. Add 50 ml of the Luciferase Assay Reagent.

6. Read with a luminometer.