Science Gateway > Protocols > Cell Biology Protocols - Table of Contents
Star Republic: Guide for Biologists

Establish stable cell clones --- drug concentrations

Geneticin (G418)
(mg/ml)

Hygromycin
(mg/ml)

Zeocin
(mg/ml)

Puromycin
(
ml/ml)

293

0.4-1.0

0.1

0.1-0.2

1.0

BHK

0.6-0.8

CHO

0.25-0.4

0.6

0.25

8.0

CHO-K1

0.5-0.8

5.0

COS

0.5-1.5

0.4

0.4

2.0

ES cells

0.25-0.4

HeLa

0.4-1.0

0.2

0.4

1.0

HepG2

0.5

0.6

0.3

Jurkat

1.0

0.8

0.5

0.5

NIH 3T3

0.4-0.5

0.2

1.0

6.0

PC12

0.4

0.5

3.0

Schneider S2

1.5

0.3