Science Gateway > Protocols > Cell Biology Protocols - Table of Contents

Pubplus.org - a PubMed search engine
Star Republic: Guide for Biologists

Adaptation to new media

1. Starting with cells that are in growth phase with at least 80% viability, sub-culture cells 1:3 to 1:2 in 75% old medium + 25% new medium.

2. Continue sub-culture in 75% old medium + 25% new medium until doubling time is comparable to that in the old medium and there is no change in the cell shape.

3. Repeat Step 1-2 in 50% old medium + 50% new medium, 25% old medium + 75% new medium, and finally 100% new medium.