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Star Republic: Guide for Biologists

Splicing signal

Virtualab Protocols (Chang Bioscience)
1. Most introns start with GU and end with AG. A small fraction of introns start with AT and ends with AC.

2. The branch site is located 20-50 bases upstream of the acceptor site.

5'-splice site
(donor)
Branch site 3'-splice site
(acceptor)
A/C A G G U Pu A G U          C U Pu A Py      Py rich    N C A G G

Pu = A or G, Py = C or U, and N = Pu or Py.