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Star Republic: Guide for Biologists

Protease inhibitors

Inhibitor Specificity Stock
Aprotinin Serine protease inhibitor. Inhibits plasmin, trypsin, and chymotrypsin. Does not inhibit thrombin or Factor X. 10 mg/ml in water. Store at -20 C. Stable for at least 1 week at 4 C.
EDTA Inhibits metalloproteases. 0.5 M in water (pH8-9). Store at 4 C. Stable at 4 C.
EGTA Ca2+-dependent proteases. 200 mg/ml in 1N NaOH. Store at 4 C. Stable at 4 C.
Leupeptin inhibits serine and cysteine proteases such as trpsin, papain, and cathepsin B. 1 mg/ml in water. Store at -20 C. Stable at least 1 week at 4 C.
Pepstatin inhibits aspartic acid proteases such as pepsin, renin, cathepsin D, chymosin. 1 mg/ml in methanol. Store at -20 C. Stable at least 1 week at 4 C.
PMSF (Phenylmethylsulfonyl fluoride) inhibits serine and cysteine proteases such as chymotrypsin, trypsin, thrombin, and papain. 10 mg/ml in isopropanol or ethanol. Store at -20 C. Stable in isopropanol, unstable in water.

To use, dilute 1:100 to 1:1000. A combination of protease inhibitors (Protease inhibitor set) are frequently used.