Science Gateway > Protocols > Cell Biology Protocols - Table of Contents
Star Republic: Guide for Biologists

0.1M Phosphate buffer chart

Stock solution A

0.2 M monobasic sodium phosphate, monohydrate (27.6g/L)

Stock solution B

0.2 M dibasic sodium phosphate (28.4 g/L).

Mixing an appropriate volume (ml) of A and B as shown in the table below and diluting to a total volume of 200 ml, to make a 0.1 M phosphate buffer of the required pH at room temperature. To make a 1 M phosphate buffer starting with 2 M A and B stocks.

0.2 M monobasic
sodium phosphate
0.2 M dibasic
sodium phosphate
pH
92.0 8.05.8
90.010.05.9
87.712.36.0
85.515.06.1
81.519.56.2
77.522.56.3
73.526.56.4
68.531.56.5
62.537.56.6
56.543.56.7
51.049.06.8
45.055.06.9
39.061.07.0
33.067.07.1
28.072.07.2
23.077.07.3
19.081.07.4
16.084.07.5
13.087.07.6
10.589.57.7
8.591.57.8