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Star Republic: Guide for Biologists

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Gel

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Dye migration
(1% agarose or 8% polyacrylamide)

DNA agarose gel

0.25% bromophenol blue, 0.25% xylene cyanol FF, 30% glycerol

6X

bromophenol blue ~ 300 bp, xylene cyanol FF ~ 4 kb

0.1% bromophenol blue, 0.1% xylene cyanol FF, 60% glycerol, 60 mM EDTA

6X

bromophenol blue ~ 300 bp, xylene cyanol FF ~ 4 kb

0.2% orange G, 0.05% xylene cyanol FF, 60% glycerol, 60 mM EDTA

6X

Orange G ~ 50 bp fragment, xylene cyanol FF ~ 4 kb

0.1% bromophenol blue, 60% glycerol, 60 mM EDTA

6X

bromophenol blue ~ 300 bp

0.2% bromophenol blue, 0.2% xylene cyanol FF, 15% Ficoll

10X

bromophenol blue ~ 300 bp, xylene cyanol FF ~ 4 kb

DNA Polyacrylamide gel

0.1% bromophenol blue, 0.1% xylene cyanol FF, 60% glycerol, 60 mM EDTA

6X

bromophenol blue ~ 45 bp, xylene cyanol FF ~ 160bp

DNA Polyacrylamide/urea

0.03% bromophenol blue, 0.03% xylene cyanol FF, 1 mM EDTA, in deionized formamide.

1-2X

bromophenol blue ~ 20 bp, xylene cyanol FF ~ 75bp

Protein Polyacrylamide gel

0.03% bromophenol blue, 240 mM Tris-HCl (pH6.8), 6%SDS, 30% glycerol, 2.3M B-mercaptoethanol

3X

0.02% bromophenol blue, 160 mM Tris-HCl (pH6.8), 4%SDS, 20% glycerol, 1.5M B-mercaptoethanol

2X

0.02% bromophenol blue, 100 mM Tris-HCl(pH6.8), 200 mM DTT, 4%SDS, 20% glycerol

2X

RNA formaldehyde-agarose gel

0.025% bromophenol blue, 0.025% xylene cyanol FF, 95% formamide, 0.025% SDS, 0.025% ethidium bromide, 0.5 mM EDTA

1-2X

bromophenol blue ~ 300 bp, xylene cyanol FF ~ 4 kb

0.25% bromophenol blue, 5X gel buffer, 2.5% formaldehyde, 30% formamide, 20% glycerol, 5 mM EDTA

5X

bromophenol blue ~ 300 bp, xylene cyanol FF ~ 4 kb