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Star Republic: Guide for Biologists

Dye migration in agarose and polyacrylamide gels

Gel

Bromophenol blue

Xylene cyanol FF

Orange G

DNA agarose

0.8%-2.5%

100-300 bp

800-4,000 bp

30-50 bp

Non-denaturing polyacrylamide gel

3.50%

100 bp

460 bp

5.00%

65 bp

260 bp

8.00%

45 bp

160 bp

12.00%

20 bp

70 bp

15.00%

15 bp

60 bp

20.00%

12 bp

45 bp

Denaturing polyacrylamide gel

5.00%

35 nucleotides

140 nucleotides

6.00%

26 nucleotides

106 nucleotides

8.00%

19 nucleotides

75 nucleotides

10.00%

12 nucleotides

55 nucleotides

20.00%

8 nucleotides

28 nucleotides