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Star Republic: Guide for Biologists

Polyclonal antibody production schedule in rodent

Week 0 Bleed pre-immune serum, 0.2 ml for mouse and 0.5 ml for rat
Immunize with 25-50 g/rabbit antigen in Complete Freund's Adjuvant .
Week 2 Immunize with 15-25 g antigen in Incomplete Freund's Adjuvant (IFA).
Week 4 Immunize with 15-25 g antigen in Incomplete Freund's Adjuvant (IFA).
Week 6 Test bleed 0.2-0.5 ml.
Immunize with 15-25 g antigen in Incomplete Freund's Adjuvant (IFA).
Test for positive production (ELISA, Western, etc.)
Week 8 Immunize with 10-15 g antigen in Incomplete Freund's Adjuvant (IFA).
Week 10 Production bleed.
Continue production bleed every four weeks.