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Star Republic: Guide for Biologists

Rabbit Polyclonal Antibody Production Schedule

Week 0 Bleed 10 ml (pre-immune serum)
Immunize with 200 g/rabbit antigen in Complete Freund's Adjuvant .
Week 1 Immunize with 100 g/rabbit antigen in Incomplete Freund's Adjuvant (IFA).
Week 2 Immunize with 100 g/rabbit antigen in Incomplete Freund's Adjuvant (IFA).
Week 3 Immunize with 100 g/rabbit antigen in Incomplete Freund's Adjuvant (IFA).
Week 4 Immunize with 100 g/rabbit antigen in Incomplete Freund's Adjuvant (IFA).
Week 5 Test bleed 20-30 ml.
Immunize with 100 g/rabbit antigen in Incomplete Freund's Adjuvant (IFA).
Test for positive production (ELISA, Western, etc.)
Week 6 1st production bleed 20-30 ml if test positive.
Immunize with 100 g/rabbit antigen in Incomplete Freund's Adjuvant (IFA).
Test for positive production (ELISA, Western, etc.) again if previous tests were negative.
Week 7 2nd production bleed 20-30 ml.
Immunize with 100 g/rabbit antigen in Incomplete Freund's Adjuvant (IFA).
Test for positive production (ELISA, Western, etc.) again if previous tests were negative.
Production may be terminated if test negative.
Week 8 3rd production bleed 20-30 ml.
Immunize with 100 g/rabbit antigen in Incomplete Freund's Adjuvant (IFA).
Week 9 4th production bleed 20-30 ml.
Immunize with 100 g/rabbit antigen in Incomplete Freund's Adjuvant (IFA).
Week 10 Terminating bleed 100-120 ml. Or the rabbit can be maintained for extra bleeds.