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Star Republic: Guide for Biologists

Chicken Polyclonal Antibody Production Schedule

Edited by Chang Zhu
Chickens produce mostly IgY in eggs (~50 mg antibody per egg).

Week 0 Collect an egg (pre-immune egg).
Immunize with 200 g/chicken IM/SQ antigen in Complete Freund's Adjuvant .
Week 1 Immunize with 100 g/chicken IM/SQ antigen in Incomplete Freund's Adjuvant (IFA).
Week 2 Immunize with 100 g/chicken IM/SQ antigen in PBS.
Week 3 Immunize with 100 g/chicken IM/SQ antigen in PBS.
Week 7 Immunize with 100 g/chicken IM/SQ antigen in PBS.
Week 4-13 Collect eggs.