Science Gateway > Protocols > Cell Biology Protocols - Table of Contents - a PubMed search engine
Star Republic: Guide for Biologists

ELISA plates

ELISA plate with enhanced protein-binding should be used.

1. Dilute the protein to be bound to the ELISA plate (e.g., IgG antibody) in PBS to the concentration of 2-10 g/ml.

2. Add 100 l to each well of the 96-well ELISA plate. Shake to cover all wells.

3. Cover the plate and incubate for 1 hour at 37 C, 2 hour at room temperature, or overnight at 4C.

4. Wash with 200 l PBS/0.05% Tween-20 per well. Repeat 3 times. Discard the washing buffer by tapping against paper towel.

5. Plates can be stored in 200 l PBS/0.05% Tween-20 at 4 C. Don't let the plate dry up.