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Restriction Endonucleases > Sal I

Sal I


 
 
 Recognition site:
 
 GTCGAC
CAGCTG
 
 Activity, Fermentas:
 
Blue+ Green+ Orange+ Red+ Yellow+ 1x Yellow+ 2x
0-20% 0-20% 100% 20-50% 0-20% 50-100%
 
 
 Activity, NEB:
 
NEBuffer 1 NEBuffer 2 NEBuffer 3 NEBuffer 4
0% 0% 50% 0%
 
 Activity, Promega:
 
 
A B C D MULTI-CORE™
<10% 10–25% 25–50% 100% <10%
 
 Recommended buffer: 
Fermentas:Buffer O+
NEB:NEBuffer Sal I + BSA 150 mM
Promega:Buffer D
 
 Incubation temperature:  37°C
 
 Heat Inactivation:  65°C for 20 minutes.
 
 Links:
 
  Sal I, Fermentas
Sal I, NEB
Promega
 
 


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