Kpn I
| |
| |
| | Recognition site:
| | | GGTAC▼C
C▲CATGG | | | | | Activity, Fermentas:
| | |
| Blue+ |
Green+ |
Orange+ |
Red+ |
Yellow+ 1x |
Yellow+ 2x |
| | 20-50% |
0-20% |
0-20% |
0-20% |
20-50% |
0-20% |
| | | |
| | Activity, NEB:
| | |
| NEBuffer 1 |
NEBuffer 2 |
NEBuffer 3 |
NEBuffer 4 |
|
| 100% |
75% |
0% |
50% |
| | |
| | Activity, Promega:
| | |
| A |
B |
C |
D |
J |
MULTI-CORE™ |
|
| 100%2 |
25–50% |
25–50% |
<10% |
100% |
75–100% |
| | |
| | Recommended buffer: | | |
| Fermentas: | Buffer Kpn I+ |
| NEB: | NEBuffer 1 + BSA 10 mM |
| Promega: | Buffer J |
| | |
| | Incubation temperature: | | |
37°C | | |
| | Heat Inactivation: | | |
No |
| |
| | Links:
| | |
Kpn I, Fermentas
Kpn I, NEB
Promega |
| |
|
|
| |
|
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